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pdcas9dnmt3a egfp  (Addgene inc)


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    Structured Review

    Addgene inc pdcas9dnmt3a egfp
    Pdcas9dnmt3a Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pdcas9+dnmt3a+plasmid/pdCas9-DNMT3A-EGFP+(Plasmid+%2371666)/pm41620608-256-17-18
    Average 93 stars, based on 33 article reviews
    pdcas9dnmt3a egfp - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Comprehensive profiling of CRISPR/dCas9 epigenome editors indicates a complex link between on and off target effects.
    Article Snippet: .. Finally, after removing the Puro from the pdCas9DNMT3A-Puro plasmid by EcoRI digestion and re-ligation, the CMV-GFP-T2A-Puro cassette was cloned on the pdCas9-DNMT3A plasmid (M3-dCas9-3A, Addgene #220237). .. The same procedure was applied to the control tool, consisting of dCas9 fused with the inactive DNMT3A (M3-dCAS9DNMT3A, Addgene #220237).

    Article Title: Systematic comparison of dCas9-based DNA methylation epimodifiers over time indicates efficient on-target and widespread off-target effects
    Article Snippet: .. Finally, after removing the Puro from the pdCas9-DNMT3A-Puro plasmid by EcoRI digestion and re-ligation, the CMV-GFP-T2A-Puro cassette was cloned on the pdCas9-DNMT3A plasmid (M3-dCas9-3A, Addgene #220237). .. The same procedure was applied to the control tool, consisting of dCas9 fused with the inactive DNMT3A (M3-dCAS9-DNMT3A, Addgene #220237).

    Clone Assay:

    Article Title: Comprehensive profiling of CRISPR/dCas9 epigenome editors indicates a complex link between on and off target effects.
    Article Snippet: .. Finally, after removing the Puro from the pdCas9DNMT3A-Puro plasmid by EcoRI digestion and re-ligation, the CMV-GFP-T2A-Puro cassette was cloned on the pdCas9-DNMT3A plasmid (M3-dCas9-3A, Addgene #220237). .. The same procedure was applied to the control tool, consisting of dCas9 fused with the inactive DNMT3A (M3-dCAS9DNMT3A, Addgene #220237).

    Article Title: Systematic comparison of dCas9-based DNA methylation epimodifiers over time indicates efficient on-target and widespread off-target effects
    Article Snippet: .. Finally, after removing the Puro from the pdCas9-DNMT3A-Puro plasmid by EcoRI digestion and re-ligation, the CMV-GFP-T2A-Puro cassette was cloned on the pdCas9-DNMT3A plasmid (M3-dCas9-3A, Addgene #220237). .. The same procedure was applied to the control tool, consisting of dCas9 fused with the inactive DNMT3A (M3-dCAS9-DNMT3A, Addgene #220237).



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